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  2. Instructions For Arctica Data Collection

Instructions for Arctica data collection

Sample preparation

Basic usage of glow discharge

  • UCSF PELCO easiGlow 91000
  • UCSF EMS 700 glow discharge

Prepare negative-stained sample

  • Make your own stain (coming soon)
  • Carbon coating grids (UCSF manual)
  • Prepare negative-stained sample (coming soon)

Prepare Cryo-EM sample at UCSF EM facility core

  • Usage of FEI Vitrobot Mark IV
  • Usage of FEI Vitrobot Mark IV - Additional Notes
  • Usage of GATAN CP3 (coming soon)

Microscope Operation Manual

Basic operation of microscopes:

  • UCSF FEI Tecnai T12
  • Arctica screening
  • Arctica data collection

Standard data collection parameters:

  • Arctica pixel size and dose table
  • Glacios pixel size and dose table
  • Krios1 pixel size and dose table
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