|
Nominal Magnification |
Pixel Size (Å/pix) Super Resolution |
Pixel Size (Å/pix) Counting mode (or after 2x FT bin) |
Dose Rate (e-/pix/s); Total Dose (e-/A2) |
Exposure time (s) |
Dose (e-/A2/frame) |
Magnification of 5 μm physical pixel (super res) |
|
64,000 |
0.685 | 1.371 |
12.0 ; 63.9 |
10 |
0.54 (0.085s frame) |
36496 (72939) |
| 105,000 | 0.4094 | 0.8189 | 16 ; 47.7 | 2 | 0.596 (0.025s frame) | 61057 (122115) |
|
130,000 |
0.332 |
0.664 |
16 ; 47.2 |
1.3 |
0.54 (0.015s frame) |
75188 (150602) |
- Approximate single particle data collection speeds at 105kx (actual speeds vary based on size of image shift groups after combining targets)
- 5x5 hole IS group (1.2/1.3um foil), physical pixel size: ~11k/24hr
- 5x5 hole IS group (1.2/1.3um foil), super res pixel size: ~8k/24hr
- 3x3 hole IS group (1.2/1.3um foil), physical pixel size: ~8k/24hr
- 3x3 hole IS group (1.2/1.3um foil), super res pixel size: ~6k/24hr
- Use iLab to reserve Krios1 time (click here for instructions) (link to iLab)
- If another magnification or dose rate necessary please contact [email protected].
- UCSF MotionCorr2 v1.3.0 will be used for real time motion correction and dose-weighting.
- Other useful protocols