| Nominal Magnification | Pixel Size (Å/pix) Super Resolution | Pixel Size (Å/pix) Counting mode (or after 2x FT bin) | Dose Rate (e-/pix/s); Total Dose (e-/A2) | Exposure time (s) | Dose (e-/A2/frame) | Magnification of 5 μm physical pixel (super res) |
| 64,000 | 0.685 | 1.371 | 12.0 ; 63.9 | 10 | 0.54 (0.085s frame) | 36496 (72939) |
| 105,000 | 0.4094 | 0.8189 | 16 ; 47.7 | 2 | 0.596 (0.025s frame) | 61057 (122115) |
| 130,000 | 0.332 | 0.664 | 16 ; 47.2 | 1.3 | 0.54 (0.015s frame) | 75188 (150602) |
- Full list of calibrated pixel sizes: http://emcoresbx.ucsfsitebuilder.acsitefactory.com/content/krios-1-pixel-size-table
- Approximate single particle data collection speeds at 105kx (actual speeds vary based on size of image shift groups after combining targets)
- 5x5 hole IS group (1.2/1.3um foil), physical pixel size: ~11k/24hr
- 5x5 hole IS group (1.2/1.3um foil), super res pixel size: ~8k/24hr
- 3x3 hole IS group (1.2/1.3um foil), physical pixel size: ~8k/24hr
- 3x3 hole IS group (1.2/1.3um foil), super res pixel size: ~6k/24hr
- Use iLab to reserve Krios1 time (click here for instructions) (link to iLab)
- If another magnification or dose rate necessary please contact [email protected].
- UCSF MotionCorr2 v1.3.0 will be used for real time motion correction and dose-weighting.
- Other useful protocols