UCSF Glacios data collection settings

Nominal Magnification

Pixel Size (Å/pix)

Super Resolution

Pixel Size (Å/pix)

Counting mode (or after 2x FT bin)

Dose Rate 

(e-/pix/s); 

Total Dose (e-/A2)

Exposure time (s)

Dose (e-/A2/frame)

Magnification of 5 μm physical pixel (super res)

34,000 (CDS) 0.603 1.207 16.5 ; 68 6 0.56 (0.05 s frame)  
43,000 (CDS) 0.474 0.949 8 ; 62 7 0.53 (0.06s frame)  

 

Last updated on 

  • If another magnification or dose rate necessary please contact [email protected]
  • UCSF MotionCorr2 v1.3.0 will be used for real time motion correction and dose-weighting.
  • Other useful protocols