UCSF Glacios data collection settings

Nominal Magnification

Pixel Size

(Å/pix)

Dose Rate

(e^-/pix/s) ; (e^-/A²/s)

Exposure Time

(total, s) ; (per frame, s)

Dose per Frame

(e^-/A²/frame)

Total Dose

(e^-/A²)

C2 %

Spot Size

54,000

0.73

16 ; 30

2.0 ; 0.02

0.60 (0.02s frame)

39.891%

All calibrated pixel sizes: http://emcoresbx.ucsfsitebuilder.acsitefactory.com/content/glacios-pixel-size-table
If another magnification or dose rate necessary please contact [email protected].
UCSF MotionCorr2 v1.3.0 will be used for real time motion correction and dose-weighting.
Other useful protocols

Getting Started with the CryoEM Core

Reserve microscope time with iLab

Prepare negative-stained sample

Prepare Cryo-EM sample

Single particle workflow with SerialEM