Sample preparation

 

Basic usage of glow discharge

Prepare negative-stained sample

Prepare Cryo-EM sample 

Basic operation of microscopes 

 
 

High-resolution data collection using UCSF cryo-EM facilities

 

Screening your grids on Arctica using SerialEM software

Collecting your data on Arctica using SerialEM software

Setting up mulit-shot-per-hole collection (Krios1)

Tomography protocol in SerialEM

Doing on-the-fly MotionCorr2 using Scipion

2D classifcation on-the-fly using cryoSPARC Live 

Titan Krios GIF tuning - ZLP center ONLY

UCSF Arctica pixel size table

UCSF Glacios pixel size table

UCSF Titan Krios 1 pixel size table / sign up guide

UCSF Titan Krios 2 pixel size table / sign up guide

Starting a cryo cycle

 

Additional useful documents

 

Caltech Getting Started in Cryo-EM, by Jensen Lab

  • This class covers the fundamental principles underlying cryo-electron microscopy starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography.  
  • Here are the links, YouTube playlist, iTunes U course, Coursera on-line course, Concepts questions (pdf)

CryoEM 101

  • Cryo-EM 101 was created by Peter Shen and Janet Iwasa at the University of Utah.  It's a media-rich curriculum to augment users’ own hands-on training. The training material will contain videos, animations, and interactive simulations that cover the major components of the cryo-EM workflow.
  • Here is the link, CryoEM101

MRC Lectures

NRAMM Workshops

UCSF Cryo-EM Enthusiasts Meeting