If you will be the last user on a microscope for the next ~16hrs or longer, please start a cryo cycle. The instructions are below, but contact David Bulkley, Glenn Gilbert or Li Wang if you have any questions:
Sample preparation
Basic usage of glow discharge
Prepare negative-stained sample
- Make your own stain (coming soon)
- Carbon coating grids (UCSF manual)
- Prepare negative-stained sample (coming soon)
Prepare Cryo-EM sample at UCSF EM facility core
- Usage of FEI Vitrobot Mark IV
- Usage of FEI Vitrobot Mark IV - Additional Notes
- Usage of GATAN CP3 (coming soon)