| Nominal Magnification | Pixel Size (Å/pix) | Dose Rate (e-/pix/s); Dose (e-/A2/s) | Exposure time (s) | Total Dose (e-/A2) 954 frames | Magnification of 14 μm physical pixel |
| 165,000 | 0.7262 | 7.5 ; 13.69 | 3.0 | 41 | 192,784 |
- Use iLab to book time for Krios2. Please email [email protected], [email protected], or [email protected] for any further modifications to grid loading and microscope/camera related issues.
- CSLive is setup for OTF patch-based motion correction, and all output can be exported to your local workstations so you do not need to repeat any steps. Please contact David with questions about how to set this up.
- The above 165kx settings were tested using a 5x5 image shift on 1.2/1.3um holey carbon. Within ~1hr of collection, a 1.55Å reconstruction of Apoferritin was obtained. Rate of data acquisition is roughly ~15k micrographs/24hr
Last updated: 10/28/2025