Tomography protocol in SerialEM

by: Arthur Melo



This protocol assumes you already have acquired an atlas (at low mag) for the entire grid and a prepMMM (at a mag where you can see the entire square) of one or multiple grid squares. The first step is to generate maps of individual squares at 4600x magnification. If you want to collect only one tilt series, skip to STEP 4.




--at this point, you are ready to run a prepMMM for the squares you want to obtain a 4600x stitched map for 


   after obtaining your individual square maps:





Tilt series schemes


1.Bidirectional tilt series
2.Dose symmetric tilt series
3.Beam Intensity or Exposure control : Keep beam intensity constant

Set up Focus and Trial

  • Put trial and focus on the tilt axis