| Nominal Magnification | Pixel Size (Å/pix) | Dose Rate (e-/pix/s); Dose (e-/A2/s) | Exposure time (s) | Total Dose (e-/A2) 954 frames | Magnification of 14 μm physical pixel |
| 165,000 | 0.7262 | 8 ; 15.17 | 3.0 | 45.5 | 192,784 |
- All pixel sizes: http://emcoresbx.ucsfsitebuilder.acsitefactory.com/content/krios-2-pixel-size-table
- Use iLab to book time for Krios2. Please email [email protected], [email protected], or [email protected] for any further modifications to grid loading and microscope/camera related issues.
- CSLive is setup for OTF patch-based motion correction, and all output can be exported to your local workstations so you do not need to repeat any steps. Setup instructions are here: http://emcoresbx.ucsfsitebuilder.acsitefactory.com/content/cryosparc-live-for-otf-motion-correction-on-krios2. Please contact David with questions about CSLive.
- The above 165kx settings were tested using a 5x5 image shift on 1.2/1.3um holey carbon. Within ~1hr of collection, a 1.55Å reconstruction of Apoferritin was obtained. Rate of data acquisition is roughly ~15k micrographs/24hr
Last updated: 02/06/2026