|
Nominal Magnification |
Pixel Size (Å/pix) Counting ; Super-res |
Dose Rate (e-/pix/s) ; (e-/A2/s) |
Exposure time (total, s) ; (per frame, s) | Dose per frame (e-/A2/frame) | Total Dose (e-/A2) | C2 % | Spot size |
|
45,000 |
0.865; 0.432 |
16 ; 21.4 | 3.0 ; 0.04 | 0.85 | 64.1 | 39.784% | 6 |
Sample preparation
Basic usage of glow discharge
Prepare negative-stained sample
- Make your own stain (coming soon)
- Carbon coating grids (UCSF manual)
- Prepare negative-stained sample (coming soon)
Prepare Cryo-EM sample at UCSF EM facility core
- Usage of FEI Vitrobot Mark IV
- Usage of FEI Vitrobot Mark IV - Additional Notes
- Usage of GATAN CP3 (coming soon)