Structural basis for recognition of the Hsp90 closed, ATP state by the TPR-containing co-chaperone FKBP51.

Alzheimer's & Dementia

Kanghyun Lee, Aye Thwin, Eric Tse, Stephanie Gates, Daniel Southworth. Structural basis for recognition of the Hsp90 closed, ATP state by the TPR-containing co-chaperone FKBP51. Alzheimer's & Dementia. 2021 Dec 1; 17(S3).

2021

https://researcherprofiles.org/profile/344527027

Kanghyun Lee, Aye Thwin, Eric Tse, Stephanie Gates, Daniel Southworth

Sample preparation

Basic usage of glow discharge

UCSF PELCO easiGlow 91000
UCSF EMS 700 glow discharge

Prepare negative-stained sample

Make your own stain (coming soon)
Carbon coating grids (UCSF manual)
Prepare negative-stained sample (coming soon)

Prepare Cryo-EM sample at UCSF EM facility core

Usage of FEI Vitrobot Mark IV
Usage of FEI Vitrobot Mark IV - Additional Notes
Usage of GATAN CP3 (coming soon)