Conformational Plasticity of the Clpap Aaa+ Protease Couples Protein Unfolding and Proteolysis.

Biophysical journal

Alexandrea N. Rizo, Kyle Eric Lopez, James Shorter, Daniel R. Southworth. Conformational Plasticity of the Clpap Aaa+ Protease Couples Protein Unfolding and Proteolysis. Biophysical Journal. 2021 Feb 1; 120(3):99a.

2021

https://researcherprofiles.org/profile/176346715

Alexandrea N. Rizo, Kyle Eric Lopez, James Shorter, Daniel R. Southworth

Sample preparation

Basic usage of glow discharge

UCSF PELCO easiGlow 91000
UCSF EMS 700 glow discharge

Prepare negative-stained sample

Make your own stain (coming soon)
Carbon coating grids (UCSF manual)
Prepare negative-stained sample (coming soon)

Prepare Cryo-EM sample at UCSF EM facility core

Usage of FEI Vitrobot Mark IV
Usage of FEI Vitrobot Mark IV - Additional Notes
Usage of GATAN CP3 (coming soon)