THE STRUCTURAL BASIS FOR POLYPEPTIDE TRANSLOCATION BY THE HSP104 DISAGGREGASE.

The FASEB Journal

Daniel R Southworth, Adam Yokom, Stephanie Gates, Meredith Jackerel, James Shorter. THE STRUCTURAL BASIS FOR POLYPEPTIDE TRANSLOCATION BY THE HSP104 DISAGGREGASE. The FASEB Journal. 2017 Apr 1; 31(S1).

2017

https://researcherprofiles.org/profile/459947363

Daniel R Southworth, Adam Yokom, Stephanie Gates, Meredith Jackerel, James Shorter

Sample preparation

Basic usage of glow discharge

UCSF PELCO easiGlow 91000
UCSF EMS 700 glow discharge

Prepare negative-stained sample

Make your own stain (coming soon)
Carbon coating grids (UCSF manual)
Prepare negative-stained sample (coming soon)

Prepare Cryo-EM sample at UCSF EM facility core

Usage of FEI Vitrobot Mark IV
Usage of FEI Vitrobot Mark IV - Additional Notes
Usage of GATAN CP3 (coming soon)