Cryo-EM Structural Analysis of Neuronal Nitric Oxide Synthase.

Reserve microscope time with iLab

• Setup your iLab account
• Booking time with iLab

Prepare negative-stained sample

• Glow discharge grids
• Carbon coat grids
• Make UF stain and prepare grids

Prepare Cryo-EM sample

• Usage of FEI Vitrobot Mark IV
• Usage of FEI Vitrobot Mark IV - Additional Notes

Single particle workflow with SerialEM

• Instructions for Arcitca screening
• Instructions for Arctica data collection