Membrane Constriction and Thinning by Sequential ESCRT-III Polymerization.

Biophysical journal

Henry C. Nguyen, Nathaniel Talledge, John McCullough, Abhimanyu Sharma, Frank R. Moss, Janet Iwasa, Michael Vershinin, Wesley I. Sundquist, Adam Frost. Membrane Constriction and Thinning by Sequential ESCRT-III Polymerization. Biophysical Journal. 2020 Feb 1; 118(3):88a.

2020
https://researcherprofiles.org/profile/151837519

Henry C. Nguyen, Nathaniel Talledge, John McCullough, Abhimanyu Sharma, Frank R. Moss, Janet Iwasa, Michael Vershinin, Wesley I. Sundquist, Adam Frost

Sample preparation

Basic usage of glow discharge

Prepare negative-stained sample

Prepare Cryo-EM sample at UCSF EM facility core