Structural and mechanistic studies of Skd3 (human CLPB) provide insight into disease-causing mutations and reveal unique AAA+ oligomerization.

Biophysical journal

Alexandrea Rizo, Ryan R. Cupo, James Shorter, Daniel R. Southworth. Structural and mechanistic studies of Skd3 (human CLPB) provide insight into disease-causing mutations and reveal unique AAA+ oligomerization. Biophysical Journal. 2022 Feb 1; 121(3):325a.

2022
https://researcherprofiles.org/profile/344527028

Alexandrea Rizo, Ryan R. Cupo, James Shorter, Daniel R. Southworth

Sample preparation

Basic usage of glow discharge

Prepare negative-stained sample

Prepare Cryo-EM sample at UCSF EM facility core