Structural basis for client engagement and chaperone cycling by human mitochondrial Hsp60.

Biophysical journal

Julian R. Braxton, Jason E. Gestwicki, Daniel R. Southworth. Structural basis for client engagement and chaperone cycling by human mitochondrial Hsp60. Biophysical Journal. 2022 Feb 1; 121(3):323a.

2022

https://researcherprofiles.org/profile/344526543

Julian R. Braxton, Jason E. Gestwicki, Daniel R. Southworth

Sample preparation

Basic usage of glow discharge

UCSF PELCO easiGlow 91000
UCSF EMS 700 glow discharge

Prepare negative-stained sample

Make your own stain (coming soon)
Carbon coating grids (UCSF manual)
Prepare negative-stained sample (coming soon)

Prepare Cryo-EM sample at UCSF EM facility core

Usage of FEI Vitrobot Mark IV
Usage of FEI Vitrobot Mark IV - Additional Notes
Usage of GATAN CP3 (coming soon)